DMEM/Nutrient Mixture F-12 Ham, With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methio

DMEM/Nutrient Mixture F-12 Ham, With 15 mM HEPES, without L-glutamine, L-leucine, L-lysine, L-methio

Stock Code: 3586133
Manufacturer Part No: D9785-10L
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£96.11 (exc VAT) per 10L
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General description


The most basic formulation offered. This formulation is used by investigators who want to start with the essential components of (1;1) DME/Ham's F-12, and have the flexibility to optimize the formula for their own application. This base formulation is particularly suitable for studies of cell protein metabolism with labeL-tagged amino acids.


Quantity


Formulated to contain 14.8 grams of powder per liter of medium.


Reconstitution


Supplement with 0.1545 g/L calcium chloride, 0.365 g/L L-glutamine, 0.05905 g/L L-leucine, 0.09125 g/L L-lysine, 0.0612 g/L magnesium chloride, 0.04884 g/L magnesium sulfate, 0.0172 g/L L-methionine, 1.2 g/L sodium bicarbonate.

ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - mammalian: suitable
Storage Temp.2-8°C
Formpowder

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