Urease Buffer Reagent,

Urease Buffer Reagent,

Stock Code: 3581507
Manufacturer Part No: U3383-1VL
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Application


Used in conjunction with Phenol Nitroprusside solution (Product Code P 6994) and Alkaline Hydpochlorite solution (Product Code A 1727) for the colorimetric determination of urea.


Urease Buffer Reagent has been used: to measure plasma urea N (PUN) by the hypochlorite-phenol nitroprusside methodin cell viability assay to measure the synthesis of urea in human fetal stem/progenitor cellsto study the metabolic capacity of rat hepatocytes


General description


Urease is a 545 kDa protein critical for the hydrolysis of urea to carbon dioxide and ammonia.


Urease is a dinickel enzyme that hydrolyses urea into ammonia and carbon dioxide. It is secreted by several bacterial species, invertebrates, fungi, and plants.Detection of urease activity is a potential diagnostic tool for Helicobacter pyloriinfections and several oral bacterial infections. two Ni2+ions bridged by a hydroxyl group and a carbamylated lysine.

Quality Level200
ManufacturerSIGMA-ALDRICH
Further InfoWhen reconstituted, product is a solution of urease from Jack bean in 27 mM EDTA with 0.1% sodium azide as a preservative.
Storage Temp.2-8°C
Formpowder

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