Trypsin from human pancreas, salt-free, lyophilized powder, vial of >=1,000 BAEE units

Stock Code: 3581438
Manufacturer Part No: T6424-1VL
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Analysis Note


Protein determined by E1%/280.


Application


Trypsin has been used in a study to assess the similarities between the hepatitis E virus and human astrovirus. Trypsin has also been used in a study to characterize a unique technique for culturing primary adult human epithelial progenitor, or stem, cells.


General description


Trypsin is a serine protease. The pancreas produce the precursor trypsinogen and secretes it to the intestine. Trypsinogen is converted to trypsin by enterokinase in the intestinal mucous membrane.


The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.


Other Notes


View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer


Quantity


Minimum 1,000 BAEE units/vial


Unit Definition


One BAEE unit will produce a δA253 of 0.001 per min at pH 7.6 at 25?°C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Formsalt-free, lyophilized powder

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