Sphingosine kinase 2 Active human, recombinant, expressed in baculovirus, >=80% (SDS-PAGE)

Sphingosine kinase 2 Active human, recombinant, expressed in baculovirus, >=80% (SDS-PAGE)

Stock Code: 3581375
Manufacturer Part No: SRP0285-20UG
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Application


Sphingosine kinase 2 is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Sphingosine kinase 2 has been used in a study to assess the expression of sphingosine kinase 1 in oestrogen receptor-negative breast cancer. Sphingosine kinase 2 has also been used in a study to investigate the antitumor effects of sorafenib (? 10 ?M) on four human neuroblastoma cell lines.


General description


Human Sphingosine kinase 2 (GenBank Accession No. AF245447), full length with N-terminal His tag, MW = 69.5 kDa, expressed in a Baculovirus infected Sf9 cell expression system.


Physical form


Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.


Preparation Note


Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.


Unit Definition


One unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min

ManufacturerSIGMA-ALDRICH
Formaqueous solution
Assay≥80% (SDS-PAGE)

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