alpha-2,6-Sialyltransferase from Photobacterium damsela, recombinant, expressed in E. coli BL21, >=5

Stock Code: 3581296
Manufacturer Part No: S2076-1UN

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Descriptions

Analysis Note

Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 8.0) containing CMP-Neu-5-Ac (1 mM) and Lac-β−OMU (1 mM) at 37 °C for 30 min and analyzed using HPLC with a fluorescence detector (excitation at 325 nm and emission at 372 nm).

Application

?-2,6-Sialyltransferase from Photobacterium damsela has been used in resialylation and restoration of sialic acids (SAs) in HRT-18G cells.

Highly active ?2-6 sialyltransferase has been used to prepare high levels of disialylated fragment crystals.

Biochem/physiol Actions

The terminal step of complexN-glycan biosynthesis is catalysed by ?-2,6-sialyltransferase (STs). Bacterial ?(2,6)-STs possesses broader acceptor substrate specificity when compared to eukaryotic ?(2,6)-STs.

Sialyltransferase transfers Neu5Ac from CMP-Neu5Ac to the galactosyl terminus of acceptor molecules including glycoproteins, glycolipids, and oligosaccharides.

General description

Human ST6Gal-I (?-galactoside ?-2,6-sialyltransferase 1) is a member of the CAZy family GT29.

Physical form

Supplied as a lyophilized powder containing Tris-HCl and NaCl.

Unit Definition

One unit will catalyze the formation of 1 µmol Neu-5-Ac-α-2,6-LacMU from CMP-Neu-5-Ac and Lac-β−OMU per minute at 37 °C at pH 8.0.