beta-Glucuronidase from Helix aspersa (garden snail), Type HA-4

Stock Code: 3579542
Manufacturer Part No: G4259-1MU
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Application


?-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospray ion trap mass spectrometry (LC/MSn) analysis, to study the structures of degradation products of baicalin.


Biochem/physiol Actions


?-glucuronidase (?-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.


Other Notes


Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis


Packaging


1000000 units in poly bottle


Unit Definition


One Sigma or modified Fishman unit will liberate 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hr at 37°C at the pH 5.0 (30 min assay).


One unit of sulfatase will hydrolyze 1.0 µmole p-nitrocatechol sulfate per hr at pH 5.0 at 37?°C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
SolubilityH2O: soluble 1.90-2.10 mg/mL, clear to slightly hazy
Formpartially purified powder

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