beta-Glucuronidase from Helix pomatia, Type H-3AF, aqueous solution, >=60,000 units/mL

beta-Glucuronidase from Helix pomatia, Type H-3AF, aqueous solution, >=60,000 units/mL

Stock Code: 3579474
Manufacturer Part No: G0762-100KU
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Application


New Technical Article Comparing Performance of Different EnzymesLearn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine


Biochem/physiol Actions


Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis


Linkage


A further processing of Type H-3 to remove agglutinin.


Physical form


Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.


Quality


Many β-glucuronidases derived from mollusks also contain sulfatase activity.


Unit Definition


One Sigma or modified Fishman unit will liberate 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hr at 37?°C at pH 5.0 (30 min assay).


Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 µmole p-nitrocatechol sulfate per hr at pH 5.0 at 37?°C.

Quality Level300
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Impurities≤1,000 unit/mL Sulfatase
Formaqueous solution

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