Alcohol Oxidase from Candida boidinii, lyophilized powder, 5-15 units/mg protein

Stock Code: 3579016
Manufacturer Part No: A6941-50UN

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Descriptions

Application

Alcohol oxidase is used to catalyze the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes . It may be used to study methanol metabolism is yeasts, such as Candida, Pichia, and Hansenula. It is useful to study protein translocation into peroxisomes. Product A6941 is from Candida boidinii and is provided as a lyophilized powder.

Biochem/physiol Actions

Alcohol oxidase has the highest affinity for methanol. The affinity decreases with increasing chain length of the alkyl (R) group. Alcohol oxidase is primarily localized in the peroxisome but is also found in the cytoplasm. Alcohol oxidase is a 600 kDa homooctomeric flavoprotein with eight equal 74 kDa subunits; each containing a flavin adenine dinucleotide (FAD) molecule. The enzyme shows little activity toward secondary, tertiary, or aromatic alcohols; or aliphatic alcohols with a chain length of more than 5 carbons. The pH range for activity of this product is 6.5-8.5, with the optimum pH being 7.5.

Packaging

50, 250 units in glass bottle

Physical form

Contains potassium phosphate buffer salts, DTE, and stabilizer

Unit Definition

One unit will oxidize 1.0?µmole of methanol to formaldehyde per min at pH?7.5 at 25?°C.

Specifications

Quality Level	200
Manufacturer	SIGMA-ALDRICH
Storage Temp.	−20°C
Solubility	100 mM potassium phosphate, pH 7.5: soluble 1.0 mg/mL at 25 °C (Cold)
Form	lyophilized powder