3,3,5,5-Tetramethylbenzidine dihydrochloride, tablet, 1 mg substrate per tablet

3,3,5,5-Tetramethylbenzidine dihydrochloride, tablet, 1 mg substrate per tablet

Stock Code: 3578783
Manufacturer Part No: T3405-100TAB
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Application


3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.


3,3?,5,5?-Tetramethylbenzidine dihydrochloride has been used as a substrate for theanti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice seraanti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysatein enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients

Quality Level200
mp≥300 °C
InChI keyNYNRGZULARUZCC-UHFFFAOYSA-N
InChI1S/C16H20N2.2ClH/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14;;/h5-8H,17-18H2,1-4H3;2*1H
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formtablet

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