4-Nitrophenyl ?-D-N,N?,N??-triacetylchitotriose has been used as a substrate for determining the endochitinase activity of a novel protein isolated from the yam (Dioscorea opposita Thunb.) (DOI), enzyme activity of recombinant chitinase CvChi45.
General description
4-Nitrophenyl ?-D-N,N?,N??-triacetylchitotriose is a substrate for hydrolytic activity of chitinase enzyme, with the release of chromogenic end product p-nitrophenol, measured at 405 nm.