Iscoves Modified Dulbeccos Medium, AQmedia, With L-alanyl-L-glutamine and sodium bicarbonate, steril

Iscoves Modified Dulbeccos Medium, AQmedia, With L-alanyl-L-glutamine and sodium bicarbonate, steril

Stock Code: 3572813
Manufacturer Part No: I2911-500ML
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£54.47 (exc VAT) per 500ML
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Application


Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.


General description


This IMDM differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures.


Legal Information


StableCell is a trademark of Sigma-Aldrich Co. LLC

Quality Level400
Pack500ML
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - mammalian: suitable, cell culture - hybridoma: suitable
Storage Temp.2-8°C
ImpuritiesEndotoxin, tested
Formliquid

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