Trypsin-EDTA solution, 1 x, sterile-filtered, BioReagent, suitable for cell culture, 500 BAEE units

Trypsin-EDTA solution, 1 x, sterile-filtered, BioReagent, suitable for cell culture, 500 BAEE units

Stock Code: 3572689
Manufacturer Part No: T4299-100ML
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£67.13 (exc VAT) per 100ML
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Application


The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. It can be used with endothelial cell cultures.


Biochem/physiol Actions


Trypsin cleaves peptides on the C-terminal side of lysine and arginine residues. The rate of hydrolysis of this reaction is slowed if an acidic residue is on either side of the cleavage site and hydrolysis is stopped if a proline residue is on the carboxyl side of the cleavage site. The optimal pH for trypsin activity is 7-9. Trypsin can also act to cleave ester and amide linkages of synthetic derivatives of amino acids. EDTA is added to trypsin solutions as a chelating agent that neutralizes calcium and magnesium ions that obscure the peptide bonds on which trypsin acts. Removing these ions increases the enzymatic activity. Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.


Caution


This product is stored frozen between -10 and -40°C. Repeated cycles of freezing and thawing should be avoided.


Components


Trypsin consists of a single chain polypeptide of 223 amino acid residues, produced by the removal of the N-terminal hexapeptide from trypsinogen which is cleaved at the Lys - lle peptide bond. The sequence of amino acids is cross-linked by 6 disulfide bridges. This is the native form of trypsin, beta-trypsin. BETA-trypsin can be autolyzed, cleaving at the Lys - Ser residue, to produce alpha-trypsin. Trypsin is a member of the serine protease family.

Quality Level400
Pack100ML
pH7.0-7.6
ManufacturerSIGMA-ALDRICH
Product LineBioReagent
ImpuritiesPorcine parvovirus, none detected (9 CFR)
Formsolution

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