LuminiCell Tracker 540Vßßßßßßßßßßßß&#223

Stock Code: 3572518
Manufacturer Part No: SCT012

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Descriptions

Application

Biocompatible organic fluorescent nanoparticles (AIEDots) that can be used to fluorescently tag vasculature in living tissues and animals for studies of inflammation and vascular leakage.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Absorbance Maximum: 423nm
Emisson Maximum: 540nm

Suggested Filters:
Excitation: 405/458/488nm
Emisson: 480-560nm

Long-term noninvasive cell tracking by fluorescent probes and quantum dots is of great importance to life science and biomedical engineering. Current methods used to fluorescently tag cells have been limited by short signal duration, high background auto-fluorescence or lengthy molecular cloning manipulations using GFP. LuminiCell Trackers﹤TMSYMBOL>﹤/TMSYM﹤WBR>BOL> are biocompatible organic fluorescent nanoparticles based on Aggregation Induced Emission technology, termed AIEDots. Aggregation induced emission (AIE) molecules emit fluorescence in an opposite manner than other common fluorophores (Quantum Dots, GFP). Propeller-shaped AIE fluorogens are non-emissive in solutions but become highly fluorescent upon aggregate formation. Due to these differences, LuminiCell Trackers﹤TMSYMBOL>﹤/TMSYM﹤WBR>BOL> have very high fluorescence intensities with minimal signal quenching allowing live cell fluorescent tagging for up to 10 days ﹤I>in vitro﹤/I> and 21 days ﹤I>in vivo﹤/I>. These properties make them optimal candidates for long interval live cell bioimaging experiments.﹤BR/>﹤BR/>The Luminicell Tracker﹤TMSYMBOL>﹤/TMSYMBOL>-540 Vascular Labeling Kit contain green fluorescent AIEDots nanoparticles without TAT sequences. These nanoparticles can be used to fluorescently tag vasculature in living tissues and animals for studies of inflammation and vascular leakage.﹤BR/>﹤BR/>﹤B>Features and Benefits﹤/B>﹤BR/>﹤BR/>? Brighter: 10X brighter than other cell labeling technologies﹤BR/>? Photostable: 3X longer fluorescence without signal quenching﹤BR/>? Biocompatible: Non-toxic organic nanoparticles intended for biological applications﹤BR/>? Rapid Protocol: Easy-to-use protocol labels cells within 4 hours

Physical form

500nM in 0.5ml PBS, pH 7.4

Quality

Absorbance: 422+-5nM
Concentration: 180-220nM
Fluorescence: 540+/- 10nm
Quantum Yield: ≥50%
Brightness at 540nM: ≥3X10^7 M-1cm-1

Storage and Stability

Store at -2-8°C upon receipt. Thaw at room temperature or in a water bath. Do not Freeze.

Note: Some particulates may form as a result of nanoparticle aggregation during shipping. To get particulates back in solution, sonicate the vial containing LuminiCell Tracker™ three times for 1 min each before use.