Poly-L-lysine hydrobromide, mol wt 30,000-70,000, lyophilized powder, gamma-irradiated, BioXtra, sui

Poly-L-lysine hydrobromide, mol wt 30,000-70,000, lyophilized powder, gamma-irradiated, BioXtra, sui

Stock Code: 3572199
Manufacturer Part No: P9155-5MG
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£104.88 (exc VAT) per 5MG
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Analysis Note


Molecular weight based on viscosity. Also assayed by MALLS.


Application


Recommended as a cell culture substratum when using 0.5 ml of a 0.1 mg/ml solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.Poly-L-lysine hydrobromide has been used to adhere the dissociated cells of cortical tissues into the culture plates. It has also been used to treat slides, to fix samples on it for immunohistochemical analysis.


Caution


Sterile solutions are stable for up to 2 years when stored at 2-8°C. It should be stored desiccated at -20°C.


Components


Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.


General description


Poly-L-lysine hydrobromide is a nonspecific attachment factor in promoting cell adhesion to the substratum.


Other Notes


For additional technical information on polyamino acids please visit the Polyamino acid FAQ resource.

Quality Level200
Pack5MG
InChI keyWBSCNDJQPKSPII-KKUMJFAQSA-N
InChI1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
ManufacturerSIGMA-ALDRICH
Product LineBioXtra
Formlyophilized powder

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