Peg solution hybri-max

Peg solution hybri-max

Stock Code: 3572184
Manufacturer Part No: P7181-5X5ML
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Application


Polyethylene glycol solution has been used in the hybridization of the following:irradiated camel fibroblast cells with A23 hamster cellsmouse splenocytes with Sp2/0-Ag 14 mouse myeloma cellsrabbit splenocytes and cell line 240E-1


Recommended for use in a normal fusion protocol requiring 50% PEG.


PEG is used as a fusogen to obtain hybridomas for monoclonal antibody production. Induces cell hybridization.


Biochem/physiol Actions


Polyethylene glycol (PEG) is used in detergents, emulsifying agents, soaps, metal costing process, pharmaceutical preparations and cosmetics. PEG is commonly used as a lubricant. It?s application is also observed in chromatographic stationary phases. PEG prevents crystal formation in emulsions.


General description


Polyethylene glycol (PEG) is a polymer of ethylene oxide and water, obtained upon condensation.


Legal Information


Hybri-Max is a trademark of Sigma-Aldrich Co. LLC


Other Notes


Contains 50% (w/v) polyethylene glycol (Av. Mol. Wt. 1450) in DPBS without calcium.


Packaging


Packaged in sealed ampules under nitrogen.


Reconstitution


Solution is ready-to-use. If a less concentrated solution is desired, dilute with sterile DPBS without calcium (D5773). Some precipitate may appear after being exposed to cooler, but should disappear as the solution warms. Solution may be frozen if desired but should first be aliquotted to avoid repeated freeze/thaw cycles.

InChI keyLYCAIKOWRPUZTN-UHFFFAOYSA-N
InChI1S/C2H6O2/c3-1-2-4/h3-4H,1-2H2
ManufacturerSIGMA-ALDRICH
Product LineBioReagent
Impuritiesendotoxin, tested
Formsolution

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