Poly-L-lysine solution, mol wt 70,000-150,000, 0.01%, sterile-filtered, BioReagent, suitable for cel

Poly-L-lysine solution, mol wt 70,000-150,000, 0.01%, sterile-filtered, BioReagent, suitable for cel

Stock Code: 3572152
Manufacturer Part No: P4707-50ML
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Application


Poly-L-lysine polymers can be used in promoting cell adhesion to solid substrates, conjugation to methotrexate for increased drug transport, microencapsulation of islets, cell microencapsulation technology, microarray glass slide coating, and chromosomal preparations. Lower molecular weight poly-L-lysine (30,000-70,000) is less viscuous in solution, but higher molecular weight versions provide more attachment sites per molecule.


Poly-L-lysine coated glass coverslips have been used for mounting samples for scanning electron microscopy.Poly-L-lysine coated wells were used for trapping of PC-12 cells on electrodes.Poly-L-lysine-coated borosilicate glass slides were used for the seeding of viral vector-transduced cells.


Biochem/physiol Actions


Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor.


Components


Poly-L-lysine is a positively charged amino acid polymer with approximately one HBr per lysine residue. The hydrobromide allows the poly-L-lysine to be in a crystalline form soluble in water. A small amount of product may be found in the beta structure because the HBr interferes with hydrogen bonding between amino and either the carboxyl groups or N or O containing moieties.


Preparation Note


This product is supplied as a 0.01% sterile filtered solution in water. Coating slides with this solution should be followed with a 5 minute incubation and drying the slides at room temperature. Coated slides will be stable for one year if protected from dust.

Quality Level300
Pack50ML
InChI keyWBSCNDJQPKSPII-KKUMJFAQSA-N
InChI1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1
ManufacturerSIGMA-ALDRICH
Product LineBioReagent
Impuritiesendotoxin, tested
Formliquid

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