Analysis Note
ControlHemopoietic cells and a wide range of normal human tissues
Application
Research CategoryStem Cell Research
FACS Analysis: a starting range of 5-20 µg/ml is suggested.
Immunoprecipitation
Immunoblotting
Optimal working dilutions must be determined by the end user.
Research Sub CategoryPluripotent & Early Differentiation
Anti-TRA-1-85 Antibody, blood group antigen Ok(a), clone TRA-1-85 detects level of TRA-1-85 & has been published & validated for use in FC, IP & WB.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
The high-frequency blood group antigen Ok(a) is carried on a red cell membrane glycoprotein (gp) of 35-69 kDa that is widely distributed on malignant cells of different origins. Immunostaining of hemopoietic cells and a range of normal human tissues demonstrated a wide distribution of the Ok(a) gp that appears to be nonlineage-restricted, although certain tissues show differentiation-related expression. This clone can be used to monitor contamination of human Embryonic Stem cell cultures with murine feeder cells.
Immunogen
Epitope: blood group antigen Ok(a)
2102Ep human Embryonal Carcinoma Cells
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Physical form
Purified Immunoglobulin. Liquid in 0.02M PBS, 0.25 NaCl ph 7.6 with 0.1% Sodium Azide
Format: Purified
Specificity
This antibody exhibits pan-human reactivity, recognizing the cell surface blood group antigen Ok (a). Reactivity to murine cells has not been observed.
SPECIES REACTIVITIES:
Refer to comments regarding specificity
Storage and Stability
Maintain refrigerated at 2-8C in undiluted aliquots for up to 12 months
Target description
35-69 kDa