MEM Non-essential Amino Acid Solution (100x), without L-glutamine, liquid, sterile-filtered, BioReag

MEM Non-essential Amino Acid Solution (100x), without L-glutamine, liquid, sterile-filtered, BioReag

Stock Code: 3571975
Manufacturer Part No: M7145-100ML
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£15.60 (exc VAT) per 100ML
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Application


Adding supplements of amino acids to media both stimulates growth and prolongs the viability of cells in culture. MEM Non-essential Amino Acid Solution supplement adds nutrients and reduces the biosynthetic burden on cell in vitro. MEM Non-essential Amino Acid Solution has been used in Dulbecco?s minimum essential media for the maintenance of mammalian cells. It has been supplemented in medium prepared for oocyte maturation.


Biochem/physiol Actions


Studies on hybridoma culture shows that addition of Eagle?s minimum essential medium (MEM) non-essential amino acid leads to enhanced membrane stability, during death phase of the culture. This also results in increased antibody secretion during death phase, and decreased lactate concentration.


General description


Eagle?s minimum essential medium (MEM) is a synthetic culture medium, which is composed of balanced salt solutions, and amino acids and vitamins. These components are essential for the growth of cultured cells. MEM might be supplemented with non-essential amino acids, to make MEM non-essential amino acid solution. The non-essential amino acids include L-alanine, L-asparagine, L-aspartic acid, L-glycine, L-serine, L-proline and L-glutamic acid.

Quality Level200
Pack100ML
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - mammalian: suitable
Product LineBioReagent
Storage Temp.2-8°C
Impuritiesendotoxin, tested
Formliquid

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