Recombinant Mouse LIF (50ug)

Recombinant Mouse LIF (50ug)

Stock Code: 3571938
Manufacturer Part No: LIF2050
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Analysis Note


Specific Activity: The activity of mouse LIF is determined by the ability to induce differentiation of murine M1 myeloid leukemic cells.The minimum detectable concentration of mouse LIF in this assay is 0.5 ng/mL


Tested negative in both aseptic and mycoplasmic tests.


Application


Research CategoryStem Cell Research


QC PROTOCOL

M1 Bioassay

1. The M1 bioassay is performed using in vitro semi-solid agar cultures, which contain approximately 300 cells in 1 mL volumes of DME containing 20 % FCS in 0.3% agar.

2. Add 100 µL of sample or mLIF (10(E4) units/mL in 5% FCS in isotonic saline) in two-fold serial dilutions in duplicate to 35 mm petri dishes.

3. Add 100 µL of 5% FCS in isotonic saline to two control slides.

4. Incubate at 37°C in fully humidified atmosphere of 10% CO2 in air for 7 days.

5. Score the number of colonies that show differentiation (note: 50 units is defined as the amount of activity which results in 50% of the colonies being differentiated).


50 µg, mouse recombinant LIF protein, expressed in E. coli, suitable for stem cell culture


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.LABEL LICENSE - USE RESTRICTED TO RESEARCH USE ONLY

Leukemia Inhibitory Factor ("LIF") is protected by US Patents No. 5,443,825 and 5,750,654 and Canada Patent No. 1,341,581 and 1,341,469 owned by AMRAD Corporation Ltd. and licensed exclusively to Millipore for research uses. The use of this product is limited for research uses only and not for any commercial purposes, such as making or developing other research products from this product or use of this product as a component in a research kit. This product is not to be used in humans or for any diagnostic purposes.

The purchaser may refuse to accept the conditions of this label license by returning this product unopened and will receive a full refund of the purchase price paid.

For-profit entities or commercial research entities purchasing this product shall be required to execute a separate commercial license with Millipore within three months of purchase. Researchers may not transfer this product or its derivatives to researchers performing commercial research at other Nonprofit Organizations, or to For-Profit Organizations, without the express written consent of Millipore, and without those entities having an express license from Millipore for the use of the product. Other than as specifically provided herein, a transferee of this product shall have no right to transfer this product, or its derivatives to any other person or entity.


General description


Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation

ManufacturerSIGMA-ALDRICH
Impurities﹤0.1 ng/mg Endotoxin (of LIF)
Formliquid
Assay>95% (SDS-PAGE)

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