ITS Liquid Media Supplement (100x), liquid, sterile-filtered, BioReagent, suitable for cell culture

ITS Liquid Media Supplement (100x), liquid, sterile-filtered, BioReagent, suitable for cell culture

Stock Code: 3571887
Manufacturer Part No: I3146-5ML
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£23.59 (exc VAT) per 5ML
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Application


ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:? mouse liver 12 (AML-12) cellsrat fetal testis explantshuman dental pulp stem cells (DPSC)


Biochem/physiol Actions


Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.Transferrin: an iron-transport protein.Selenium: an essential trace element normally provided by serum.


Components


Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 µg/ml sodium selenite at the 100x concentration.


Other Notes


ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.


Preparation Note


Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Quality Level500
Pack5ML
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - mammalian: suitable
Product LineBioReagent
Storage Temp.2-8°C
Impuritiesendotoxin, tested
Formliquid

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