IL-10;Human Recombinant Animal Free;10ug

IL-10;Human Recombinant Animal Free;10ug

Stock Code: 3571815
Manufacturer Part No: GF325
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Application


Research CategoryStem Cell Research


Research Sub CategoryGrowth Factors & Receptors


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes. IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-α. Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions. Human IL-10 active on murine cells, but murine IL-10 is inactive on human cells. Recombinant human IL-10 is an 18.6 kDa protein of 161 amino acid residues.


Product Source: Protein is expressed in E.coli.
The protein is manufactured without the use of any animal products/reagents and is considered Animal-Free and can be used in protocols where possible transmisson of animal viruses and contamination must be avoided.


Physical form


Product is presented in 20 mM Sodium Phosphate, pH 7.2. and is filtered through a 0.2 micron filter before lyophilization.


Quality


The ED50 was determined by the dosedependent co-stimulation (with human IL-4) of MC/9 cells. The expected ED50 is ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 10^5
units/mg.


Storage and Stability


Store at -20°C for up to 4 months from date of receipt Centrifuge the vial prior to opening.
Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein and store in working aliquots at -20°C.

Quality Level100
ManufacturerSIGMA-ALDRICH
Impurities﹤0.1 µg/mg protein endotoxin (<1 EU/µg)

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