Recombinant Human Wnt-1; 10ug

Recombinant Human Wnt-1; 10ug

Stock Code: 3571785
Manufacturer Part No: GF175
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£386.25 (exc VAT) per EACH
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Application


Research CategoryStem Cell Research


Research Sub CategoryGrowth Factors & Receptors


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


Wnt-1 is a secreted protein that signals through the Frizzled family of cell surface receptors and is required for normal embryonic development. Wnt-1 activation induces a complex signaling cascade that ultimately leads to the increased expression of over fifty genes. An important component of Wnt-1 signaling is the stabilization, and resulting accumulation, of the intracellular signaling protein, β-catenin. Wnt signaling induces and maintains the transformed phenotype and, in certain embryonic cell lines, supports self renewal in the absence of significant differentiation. Elevated levels of Wnt proteins are associated with tumorigenesis and are present in numerous human breast cancers. Mature human Wnt-1 is a glycosylated protein containing 343 amino acid residues. Recombinant human Wnt-1 is a 38.4 kDa, non-glycosylated protein containing 343 amino acid residues.


Product Source: E. coli.


Physical form


Product is filtered through a 0.2 micron filter before lyophilization. Product is presented in .


Quality


Bioactivity assay: The ED50 was determined by its ability to enhance BMP-2 induced alkaline phosphatase production by murine ATDC5 cells. The expected ED50 for this effect is 1.5 - 2.5 ng/ml in the presence of 200 ng/ml of human BMP-2.


Specificity


Cross ReactivtyMouse


Storage and Stability


Store at -20°C for up to 6 months from date of receipt.

Quality Level100
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - stem cell: suitable
Impurities﹤0.1 µg/mg protein endotoxin (<1EU/µg)
Assay>98% (SDS-PAGE and HPLC)

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