Wnt-3a Recombinanat Protein; 5ug

Wnt-3a Recombinanat Protein; 5ug

Stock Code: 3571772
Manufacturer Part No: GF154
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Analysis Note


Specific Activity: Wnt3a protein has been shown to be bioactive as determined by TCF/LEF-luciferase reporter. The ED50 for this effect is typically ﹤250 ng/mL. Optimal concentrations should be determined for each application.


Application


The recombinant murine Wnt-3a protein is a monomeric glycoprotein containing 328 amino acid residues.


Research CategoryStem Cell Research


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


Product Source: Mouse L cells expressing full length Wnt3a.


Recombinant murine Wnt-3a is a monomeric glycoprotein containing 328 amino acid residues. When analyzed by SDS-PAGE under non-reducing conditions, the murine Wnt-3a migrates at an apparent molecular weight of approximately 38.0-41.0 kDa.

Wnt-3a signalling has been implicated in the control of differentiation of stem cells. Wnt proteins bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase. Wnt proteins have been shown to regulate cell-to-cell interactions during embryogenesis, more specifically regulating mesoderm differentiation and osteogenesis. Wnt proteins have also been shown to have putative roles in the regulation of adult stem cells.


Linkage


Replaces: GF160


Physical form


Lyophilized from a solution containing PBS and 1.0% CHAPS buffer.


Storage and Stability


Maintain the lyophilized material at -20°C up to six months from date of receipt. Resuspend in sterile water to a concentration of 100µg/mL. Reconstituted Wnt3a should be stored in single use aliquots at -80°C for up to three months. Avoid repeated freeze/thaw cycles. During shipment, some amount of product will occasionally dispersed. It's recommended to gently tape the vial on the hard surface before reconstitution.

Quality Level100
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - mammalian: suitable
Impurities﹤0.1 ng/µg endotoxin (1EU/µg)

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