Milli-Mark Anti-TRA-2-49; clone TRA-2-49

Stock Code: 3571694
Manufacturer Part No: FCMAB133F

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Descriptions

Analysis Note

Control2102 Ep cells.

Application

Research CategoryStem Cell Research

Working Solution	Cells / test	Total Reaction Volume
5 µl	5 x 10⁵ Cells / 95 µl PBS	100 µl
10 µl	1 x 10⁶ Cells / 90 µl PBS	100 µl

Detect TRA-2-49 using this Milli-Mark Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate validated for use in FC.

Research Sub CategoryPluripotent & Early Differentiation

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

In humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.

Immunogen

Epitope: Unknown

2102Ep human Embryonal Carcinoma Cells

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Protein A purified

Purified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Quality

Evaluated by flow cytometry using 2102 Ep cells.

Specificity

Antibody recognizes TRA-2-49.