RT ESGRO; 10 MILLION UNITS/1 ML

RT ESGRO; 10 MILLION UNITS/1 ML

Stock Code: 3571636
Manufacturer Part No: ESG2207
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Analysis Note


Specific Activity: Rat ESGRO® is assessed both on murine ES {embryonic stem} cells (MBL-1) and on murine M1 myeloid leukemic cells.A standard of 50 Units is defined as the concentration of Rat ESGRO™ in 1.0mL of tissue culture medium that induces the differentiation of 50% of M1 colonies.106 units is equivalent to approximately 10 µg of pure protein, and is sufficient to treat 1L of ES cell culture media.Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mLMurine myeloid leukemic, M1 Assay: Specific Activity >108 units/mg


Application


Applications for Rat ESGRO™ include use as a reagent for the in vitro maintenance of rat ES cells. Studies have demonstrated that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF (Takahama et al. 1998).

Rat ES Cells: RtLIF produced from a transfected cell line, has been shown to effectively maintain the undifferentiated phenotype of rat ES cells when used at a concentration of 1000-1500 units / mL (Takahama et al. 1998).

At this recommended concentration 106 units of ESGRO™ is sufficient for 1L of tissue culture media.

Visit www.esgro-lif.com for additional information


ESGRO Leukemia Inhibitory Factor (LIF) supplement for rat ES cell culture. Each vial contains 10^7 units/ml


Caution


Note: LIF should not be allowed to come in contact with ruminant or swine.


Disclaimer


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


General description


Product Source: rtLIF is expressed in E. coli as a fusion protein with GST using the pGEX expression system, cleaved from GST moiety with thrombin and purified by ion-exchange chromatography.


Applications for Rat ESGRO™ include use as a reagent for the in vitro maintenance of rat ES cells. Studies have demonstrated that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF.


Legal Information


CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


ESGRO is a registered trademark of Merck KGaA, Darmstadt, Germany


Packaging


1 mL


Physical form


Format: Purified


Purified, sterile liquid. Rat ESGRO™ is supplied in liquid form as 107 Units ESGRO™ in 1.0 mL of 50mM sodium phosphate/1mM DTT / 10% glycerol / 250mM NaCl with 1% w/v bovine serum albumin BSA as a carrier for stability.

Rat ESGRO™ is supplied 0.22 micron sterile filtered, and tested negative for mycoplasma.


Quality


The highest standards of quality control are used in the manufacture of this product

Quality Level100
ManufacturerSIGMA-ALDRICH
Technique(s)cell culture - stem cell: suitable
Formliquid
Assay>85% (active component rat LIF (rtLIF), SDS-PAGE)

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