ECF Substrate

Stock Code: 1669610
Manufacturer Part No: RPN5785

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Descriptions

Alkaline phosphatase dephosphorylates ECF Substrate to produce a fluorescent product, which localizes at the site of catalysis. Product fluoresces at 540 to 560nm when excited at the appropriate wavelength.

A fluorescent substrate for alkaline phosphatase-based detection of protein blots, for use with fluorescence scanning equipment such as Storm instruments

For use in western blotting, dot, and slot blotting applications

Sensitivity comparable to chemiluminescence

The wider dynamic range of fluorescence scanning systems compared with film allows visualisation and quantitation of weak and dark bands in the same scan

Eliminates need for darkroom and film

The following reagents are provided, sufficient for detecting 2500cm² membrane: 36mg ECF substrate and 60mL dilution buffer