Amersham ECL Western Blotting System

Stock Code: 1669603
Manufacturer Part No: RPN2108
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£377.64 (exc VAT) per EACH
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Amersham ECL Western blotting detection kit uses horseradish peroxidase (HRP) conjugated anti-mouse and anti-rabbit antibodies for luminol-based detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRP-conjugated secondary antibodies. Addition of ECL detection reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera. ECL Western blotting system is the world's most widely used and referenced chemiluminescent immunodetection system.


Convenient kit that includes ECL detection reagent, HRP-conjugated antibodies, and blocking agent.


Detection of mouse and rabbit primary antibodies on western blots using HRP-conjugated secondary antibodies and ECL reagents.


Optimized system provides rapid, sensitive results using simple protocols.


Designed for use with nitrocellulose and PVDF membranes.


High sensitivity: Up to 10 times more sensitive than colorimetric methods.


Rapid: results can be rapidly obtained on X-ray film, providing hard copy data and avoiding fading associated with colorimetric detection methods.


Easy reprobing: save time and materials by reprobing on membranes up to 10 times.


Quantifiable: linear response in the range 0.2 to 2 OD units using pre-flashed Hyperfilm ECL,


Non-radioactive-no specialised facilities needed


Proven performance-the most widely referenced chemiluminescent detection method with hundreds of publications worldwide

ManufacturerCYTIVA

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