4-Nitrophenyl N-acetyl-beta-D-glucosaminide, >=99% (TLC)

4-Nitrophenyl N-acetyl-beta-D-glucosaminide, >=99% (TLC)

Stock Code: 3578634
Manufacturer Part No: N9376-250MG
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£143.46 (exc VAT) per 250MG
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Application


N-acetyl-?-D-glucosaminidase (NAG) has been used:as a substrate to assay N-acetyl-?-D-glucosaminidase (NAG)as a substrate to study chitinase ChiB1 activityto assess degranulation using rat basophilic leukemia (RBL)-2H3 cells


Biochem/physiol Actions


4-Nitrophenyl N-acetyl-?-D-glucosaminide (NP-GlcNAc) is a widely used hexosaminidase substrate. Enzymatic action cleaves the glycosidic bond and forms 4-nitrophenolate (pNP) which is measured at 405 nm. NP-GlcNAc can also be used in combination with diethylaminoethyl-a-cyclodextrin (DEn-CD) for a rapid and accurate rate assay method for b-N-acetylglucosaminidase. The cyclodextrin derivative is used as an additive to ionize 4-nitrophenol to yellow-colored 4-nitrophenoxide at a pH near 5, the pH optimum for the enzyme.


Packaging


1 g in poly bottle


100, 250, 500 mg in poly bottle


Substrates


Chromogenic substrate for N-acetyl-glucosaminidase.

Quality Level300
InChI keyOMRLTNCLYHKQCK-DHGKCCLASA-N
InChI1S/C14H18N2O8/c1-7(18)15-11-13(20)12(19)10(6-17)24-14(11)23-9-4-2-8(3-5-9)16(21)22/h2-5,10-14,17,19-20H,6H2,1H3,(H,15,18)/t10-,11-,12-,13-,14-/m1/s1
ManufacturerSIGMA-ALDRICH
Impurities≤0.05% Free p-nitrophenol
Solubilitywater: 5 mg/mL, clear, colorless to very faintly yellow
Formpowder
Assay≥99% (TLC)

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