3-Phosphoglyceric Phosphokinase from bakers yeast (S. cerevisiae), ammonium sulfate suspension, >=10

Stock Code: 3581191
Manufacturer Part No: P7634-5KU
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Analysis Note


Protein determined by TCA Biuret.


Application


3-Phosphoglyceric Phosphokinase generates ATP by catalyzing the transfer of a phosphate group from 1,3-diphosphoglycerate to ADP. 3-Phosphoglycerate Phosphokinase is used to study glycolysis and gluconeogenesis. It has also been used to study low molecular weight GTP-binding proteins and mechanisms of inhibition of glyceraldehyde-3-phosphate dehydrogenase.


The enzyme has been used in the assay of glyceraldehyde-3-phosphate dehydrogenase.


Biochem/physiol Actions


3-Phosphoglyceric Phosphokinase catalyzes the reversible transfer of a phosphate group from 1,3-diphosphoglycerate to ADP to generate ATP and 3-phosphoglycerate. 3-Phosphoglycerate Phosphokinase activity is essential for glycolysis and gluconeogenesis.


Physical form


Crystalline suspension in 3.0 M (NH4)2SO4 and 0.04 M tetrasodium pyrophosphate solution, pH 8.0


Unit Definition


One unit will convert 1.0 µmole of 1,3-diphosphoglycerate to 3-phosphoglycerate per min at pH 6.9 at 25 °C.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.2-8°C
Formammonium sulfate suspension

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