Acrylamide/Bis-acrylamide, BioReagent, for molecular biology, 19:1 (ratio)

Acrylamide/Bis-acrylamide, BioReagent, for molecular biology, 19:1 (ratio)

Stock Code: 3586555
Manufacturer Part No: A2917-100ML
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£31.78 (exc VAT) per 100ML
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Application


Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.


Acrylamide/Bis-acrylamide has been used:in the preparation of urea polyacrylamide gel during a modified cross-linking affinity purification protocol (cCLAP) in microsatellite genotyping and nonfluorescent elastin (ELN) (GT)n polymorphism genotypingin polyacrylamide gel for separation of proteins in SDS-PAGE for western blotting


Features and Benefits


Eliminates the need to weigh toxic acrylamide and bis-acrylamide.


General description


Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.


Packaging


1 L in poly bottle


100 mL in poly bottle


Reconstitution


To make up 100 ml of A2917, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution. For the 1 Liter size, use 630 mL of water.


Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.

Quality Level200
InChI keyOCQZXMCGTAWGEQ-UHFFFAOYSA-N
InChI1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
ManufacturerSIGMA-ALDRICH
Product LineBioReagent
Formpowder

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