Trypsin inhibitor from Glycine max (soybean), lyophilized powder

Stock Code: 3586237
Manufacturer Part No: T9128-500MG

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Descriptions

Analysis Note

One mg of trypsin inhibitor will inhibit a minimum of 1.0 mg Trypsin of activity ~10,000 BAEE units/mg trypsin.

Biochem/physiol Actions

This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.

Components

The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.

Packaging

500 mg in poly bottle

1, 5, 10 g in poly bottle

Preparation Note

The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet. Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.

Unit Definition

One trypsin unit = A₂₅₃ of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.

One trypsin unit will produce a δA₂₅₃ of 0.001 per min with BAEE as substrate at pH?7.6 at 25?°C; reaction volume 3.2?ml, 1 cm light path.

Specifications

Quality Level	300
Manufacturer	SIGMA-ALDRICH
Technique(s)	inhibition assay: suitable
Storage Temp.	2-8°C
Solubility	concentrate: >10 mg/mL, hazy, amber-yellow, phosphate buffer: 10 mg/mL, serum-free medium: soluble, balanced salt solution: 1 mg/mL, water: 10 mg/mL
Form	lyophilized powder