Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides, lyophilized powder, 550-1,100 unit

Stock Code: 3579567
Manufacturer Part No: G5885-1KU
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Application


Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used along with hexokinase in the determination of glucose from mice liver samples.


Biochem/physiol Actions


Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.


Glucose-6-Phophate Dehydrogenase (G-6-PDH) can utilize either nicotinamide adenine dinucleotide phosphate (NADP+) or NAD+ as coenzyme making it crucial for bacterial metabolism.


General description


Glucose-6-Phophate Dehydrogenase (G-6-PDH) comprises His-Asp catalytic dyad and exists as a homodimer. Structurally, G-6-PDH encompasses a Rossmann dinucleotide binding fold in the coenzyme binding domain region. It also harbors a large ? + ? domain and has a unique aspartate residue at position 374.


Packaging


10000 units in glass bottle


200, 1000, 2000 units in serum bottle


Physical form


Lyophilized powder containing Ficoll and Tris buffer salts


Unit Definition


One unit will oxidize 1.0 µmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NAD at pH 7.8 at 30 °C.

Quality Level300
ManufacturerSIGMA-ALDRICH
CompositionProtein, 15-40% biuret
Formlyophilized powder

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