SIGMAFAST p-Nitrophenyl phosphate Tablets, tablet, To prepare 20 mL

Stock Code: 3578544
Manufacturer Part No: N2770-5SET
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Application


Developed for use as a soluble substrate for the detection of alkaline phosphatase activity in Enzyme Immunoassays (EIA). pNPP is the substrate of choice in high sensitivity alkaline phosphatase-based EIA assays.One pNPP tablet and one Tris buffer tablet are dissolved in deionized water and provide a ready-to-use substrate. Each Sigma FAST pNPP tablet set yields a solution containing 1.0 mg/ml pNPP, 0.2 M Tris buffer. EIA applications using pNPP may be read in timed assays or stopped with 3N NaOH for delayed readings. SIGMAFAST require only the addition of distilled or deionized water to prepare an active substrate solution. Tablets are individually packaged in foil packets.


Legal Information


SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC


Reconstitution


Each tablet set dissolved in 20 mL deionized water yields a ready-to-use buffered solution containing pNPP.

Quality Level200
ManufacturerSIGMA-ALDRICH
Storage Temp.−20°C
Solubilitywater: 0.25 tablet/mL, clear, colorless
Formtablet

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