Dulbeccos Phosphate Buffered Saline, Modified, without calcium chloride and magnesium chloride, powd

Stock Code: 3586122
Manufacturer Part No: D5652-10X1L

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Descriptions

Application

Dulbecco?s Phosphate Buffered Saline has been used:as one of the pseudo-physiological solutions to study the in vitro degradation behavior of Fe–20Mn–1.2C alloyto dissolve Angelica gigas powder for microencapsulationto wash cells as part of cell culture treatments, wound-healing assay, transwell assay and apoptosis assayto wash amniotic membrane for cellular analyses

D-PBS is used to wash cells during preparation and serial transfer.

Biochem/physiol Actions

Dulbecco′s Phosphate Buffered Saline serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. It hydrates cells and acts as a source of bulk inorganic ions essential for normal cell metabolism. This buffered saline acts as the principal energy source for cell metabolism when combined with a carbohydrate, such as glucose. It provides a buffering system to maintain the medium within the physiological pH range.

General description

Dulbecco′s Phosphate Buffered Saline is a balanced salt solution (BSS) widely used in tissue culture.

Other Notes

This D-PBS is formulated without Ca or Mg. Formulations with Ca and Mg added are also available

Quantity

Formulated to contain 9.6 grams of powder per liter of medium.

Specifications

Quality Level	500
pH	7.2-7.8
Manufacturer	SIGMA-ALDRICH
Technique(s)	cell culture - mammalian: suitable
Storage Temp.	2-8°C
Solubility	water: 9.6 g/L, clear
Form	powder