Trypsin inhibitor from Glycine max (soybean), powder, BioReagent, suitable for cell culture

Trypsin inhibitor from Glycine max (soybean), powder, BioReagent, suitable for cell culture

Analysis Note

One mg of trypsin inhibitor will inhibit 1.0-3.0 mg trypsin of activity ~10,000 BAEE units per mg protein.

Application

This product has been tested for cell culture applications. Trypsin has been used in a study to assess the potential application in animal cell culture of an alkaline protease from a ton-toxigenic mangrove isolate of Vibri sp. V26. Trypsin has also been used in a study to improve the detection of fungi in eosinophilic mucin.

Biochem/physiol Actions

This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.

Components

The soybean trypsin inhibitor is a monomeric protein containing 181 amino acid residues in a single polypeptide chain crosslinked by two disulfide bridges.

Packaging

25, 100, 5×100, 250, 500 mg in glass bottle

1, 5 g in glass bottle

Preparation Note

The trypsin inhibitor is soluble in water and phosphate buffers at 10 mg/mL. It is soluble in balanced salt solutions at 1 mg/mL and in serum-free media. Concentrated solutions greater than 10 mg/mL may be hazy and have a yellow to amber color. After trypsinizing cells, resuspend in 1 mL trypsin inhibitor solution at 1 mg/mL for every mL of trypsin solution used for dissociation. The cell suspension should then be centrifuged at 1000 rpm, forming a cell pellet. Solutions can retain activity when stored short-term at 2-8° C. Solutions are stable in frozen aliquots at -20°C.

Unit Definition

One trypsin unit = A253 of 0.001 per minute with N-alpha-benzoyl-L-arginine ethyl ester (BAEE) as substrate at pH 7.6 at 25 °C.

One trypsin unit will produce a δA253 of 0.001 per min with BAEE as substrate at pH 7.6 at 25 °C; reaction volume 3.2 ml, 1 cm light path.

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Code Description Quality Level Pack Manufacturer Product Line Solubility Form Price Quantity
3377648 Trypsin inhibitor from Glycine max (soybean), powder, BioReagent, suitable for cell culture 300 5G SAFC BioReagent
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3572711 Trypsin inhibitor from Glycine max (soybean), powder, BioReagent, suitable for cell culture 300 100MG SIGMA-ALDRICH BioReagent
£202.50 (exc VAT) per 100MG
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3572712 Trypsin inhibitor from Glycine max (soybean), powder, BioReagent, suitable for cell culture 300 1G SIGMA-ALDRICH BioReagent
£938.75 (exc VAT) per 1G
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3572713 Trypsin inhibitor from Glycine max (soybean), powder, BioReagent, suitable for cell culture 300 250MG SIGMA-ALDRICH BioReagent
£437.50 (exc VAT) per 250MG
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3572714 Trypsin inhibitor from Glycine max (soybean), powder, BioReagent, suitable for cell culture 300 25MG SIGMA-ALDRICH BioReagent
£73.63 (exc VAT) per 25MG
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3572715 Trypsin inhibitor from Glycine max (soybean), powder, BioReagent, suitable for cell culture 300 500MG SIGMA-ALDRICH BioReagent
£837.50 (exc VAT) per 500MG
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3572716 Trypsin inhibitor from Glycine max (soybean), powder, BioReagent, suitable for cell culture 300 - SIGMA-ALDRICH BioReagent
£837.50 (exc VAT) per EACH
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