Trypsin-chymotrypsin inhibitor from Glycine max (soybean), lyophilized powder

Descriptions

Analysis Note

1 mg protein will inhibit ≥0.5?mg trypsin with activity of ~10,000 BAEE units per mg protein or ≥1.0 mg chymotrypsin with activity of ~40 BTEE units per mg protein.

Application

Trypsin-chymotrypsin inhibitor from Glycine max (soybean) has been used for flow cytometric separase activity assay. It has also been used to inhibit trypsin and chymotrypsin for in vitro gastrointestinal (GI) digestion studies.

Bowman Birk protease inhibitor prevents radiation-induced carcinogenesis by a reduction of incorrect DNA repairs, resulting in a reduced amount of dicentric chromosomes.

General description

Highly cross-linked with seven disulfide bridges, Bowman-Birk inhibitor has spatially distinct domains for trypsin and chymotrypsin inhibition.

Trypsin-chymotrypsin inhibitor belongs to the family of plant protease inhibitors. It is mainly found in the legume (Fabaceae) and cereal (Poaceae) families. Trypsin-chymotrypsin inhibitor consists of many internal disulfide bonds between conserved Cys residues, which maintains structural stability and active conformation of the inhibitory loops. Trypsin-chymotrypsin has anticarcinogenic properties.

Other Notes

View more information on Trypsin Inhibitor.

Packaging

10 mg in glass bottle

25 mg in poly bottle

50, 100, 500 mg in glass bottle

Physical form

Lyophilized powder containing phosphate buffer salts, pH 7.6

Unit Definition

One trypsin unit = δA₂₅₃ of 0.001 per min with BAEE as substrate at pH 7.6 at 25?°C. Reaction volume = 3.2 mL (1 cm light path).

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Composition	Storage Temp.	Form	Price	Quantity
3586243	Trypsin-chymotrypsin inhibitor from Glycine max (soybean), lyophilized powder	200	SIGMA-ALDRICH	Protein, 70-90% biuret	2-8°C	lyophilized powder	£598.93 (exc VAT) per 100MG	- +
3586244	Trypsin-chymotrypsin inhibitor from Glycine max (soybean), lyophilized powder	200	SIGMA-ALDRICH	Protein, 70-90% biuret	2-8°C	lyophilized powder	£104.19 (exc VAT) per 10MG	- +
3586245	Trypsin-chymotrypsin inhibitor from Glycine max (soybean), lyophilized powder	200	SIGMA-ALDRICH	Protein, 70-90% biuret	2-8°C	lyophilized powder	£183.86 (exc VAT) per 25MG	- +
3586246	Trypsin-chymotrypsin inhibitor from Glycine max (soybean), lyophilized powder	200	SIGMA-ALDRICH	Protein, 70-90% biuret	2-8°C	lyophilized powder	Click Add to Quote for price and delivery	- +
3586247	Trypsin-chymotrypsin inhibitor from Glycine max (soybean), lyophilized powder	200	SIGMA-ALDRICH	Protein, 70-90% biuret	2-8°C	lyophilized powder	£332.89 (exc VAT) per 50MG	- +