Tris(2-carboxyethyl)phosphine, immobilized on Agarose CL-4B,

Tris(2-carboxyethyl)phosphine, immobilized on Agarose CL-4B,

Analysis Note

Bio-testsGel has an effective TCEP concentration ≥8 µmol/mL gel

Application

Tris(2-carboxyethyl)phosphine, immobilized on agarose CL-4B may be used in preliminary off-line experiments to study its ability to cleave disulfide bonds by both under flow conditions using sequential injection analysis (SI). It may also be used as a reducing agent in bio-tests for disrupting the disulfide bonds within and between the proteins.

Biochem/physiol Actions

Tris(2-carboxyethyl)phosphine also referred to as TCEP reduces the disulfides by the usual mechanism of reduction of disulfides by phosphines in water. Tris(2-carboxyethyl)phosphine, immobilized on Agarose CL-4B,Trialkylphosphines (TCEP) are highly effective agents for reducing disulfide bonds in proteins, peptides and other disulfide bondcontaining molecules and are relatively non-reactive toward other functional groups. The trialkylphosphine TCEP was first described by Levison et al. as an odorless and efficient reductant of alkyl disulfides over a wide pH range. TCEP is stable in aqueous solutions and does not undergo the rapid oxidation that often occurs with other reducing agents such as dithiotreitol (DTT) and ?-mercaptoethanol (BME, 2-ME). TCEP does not interfere with commonly used sulfhydryl-reactive reagents (e.g., maleimide crosslinkers). Nevertheless, many protocols require recovery of the reduced sample separate from the reducing agent. Our Tris(2-carboxyethyl)phosphine, immobilized on Agarose CL-4B eliminates the need to use laborious and troublesome gel filtration methods to separate the reduced sample from the reducing agent. Immobilized TCEP Disulfide Reducing Gel may be adapted conveniently to a variety of scales and formats. Examples are given for batch, spin cup column and gravity-flow column procedures. For small-scale reductions, the most complete sample recovery is made using the spin-cup column procedure (see Related Thermo Scientific Products Section).

No detailed specifications are available for this product.

There are no downloads for this product.

Code Description Quality Level Manufacturer Storage Temp. Price Quantity
3586517 Tris(2-carboxyethyl)phosphine, immobilized on Agarose CL-4B, 100 SIGMA-ALDRICH 2-8°C
£178.29 (exc VAT) per 1ML
-
+
3586518 Tris(2-carboxyethyl)phosphine, immobilized on Agarose CL-4B, 100 SIGMA-ALDRICH 2-8°C
£704.79 (exc VAT) per 5ML
-
+