TRANSCREENER(TM) UDP TRFRET ASSAY

TRANSCREENER(TM) UDP TRFRET ASSAY

General description

The Transcreener® UDP2 TR-FRET Red Assay is a competitive immunoassay for UDP with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. Because it is highly selective for UDP, the assay can be used with any enzyme that generates UDP from a UDP-sugar donor. Examples include glycosyltransferase, galactosyltransferase, glucuronyltransferase, N-acetylglucosamyltransferase, N-acetylgalactosyltransferase, xylosyltransferase, and glycogen, cellulose, lactose, and hyaluronan synthases. The Transcreener® assay is designed specifically for high-throughput screening (HTS), with a single-addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener® HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families. The Transcreener® UDP2 TR-FRET Red Assay provides the following benefits: •Accommodates UDP-sugar donor concentrations ranging from 1 μM to 1,000 μM. •Excellent data quality (Z’ = 0.7) at low substrate conversion (typically 10–30%). •Overcomes the need for time-consuming, one-off assay development for individual members within an enzyme family by using a single set of assay reagents that detect an invariant product. •Time-gated detection method largely eliminates interference that can result from prompt fluorescence of test compounds. •Far-red tracer further minimizes interference from fluorescent compounds and light scattering.View full Transcreener® product list

Legal Information

Transcreener is a registered trademark of BellBrook Labs

Physical form

Kit with buffered aqueous solutions

Quantity

3022-1K = 1,000 assay, 384-well3022-10K = 10,000 assay, 384-well

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Code Description Manufacturer Storage Temp. Price Quantity
3581755 TRANSCREENER(TM) UDP TRFRET ASSAY SIGMA-ALDRICH −20°C
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3581756 TRANSCREENER(TM) UDP TRFRET ASSAY SIGMA-ALDRICH −20°C
£1,125.43 (exc VAT) per EACH
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