Taq DNA Polymerase from Thermus aquaticus, with 10x PCR reaction buffer containing MgCl2

Taq DNA Polymerase from Thermus aquaticus, with 10x PCR reaction buffer containing MgCl2

Application

Taq DNA Polymerase fromThermus aquaticus has been used:in the quantification of fungal growth by polymerase chain reaction (PCR) and photometric assayin conventional reverse transcriptase (RT)-PCRin simple sequence repeats (SSR) genotypingas a component of PCR mix for amplification of genomic and mitochondrial DNAin direct tetra-primer amplification refractory mutation system (T-ARMS) PCR to amplify dried whole blood samples

Biochem/physiol Actions

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.

Features and Benefits

Low per unit cost of Taq

General description

Taq DNA Polymerase is a thermostable enzyme derived from the thermophilic bacterium Thermus aquaticus. The enzyme is in a recombinant form, expressed in E. coli. It can withstand repeated heating to 95 °C without significant loss of activity. Each lot of Taq DNA Polymerase is tested for PCR amplification and double-stranded sequencing.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.

Other Notes

Taq DNA Polymerase is a specialized thermostable enzyme isolated from the thermophilic bacterium Thermus aquaticus. The recombinant form of this enzyme is expressed in E. coli. This 94 kDa protein shows no detectable levels of contaminating endonucleases or exonucleases by SDS-PAGE. It has both 5′→3′ polymerase and exonuclease activity.

Packaging

Taq DNA Polymerase with 10× reaction buffer containing MgCl2

Taq DNA polymerase comes with the choice of an optimized 10× reaction buffer including MgCl2 (D1806) or a 10× reaction buffer without MgCl2 plus a separate tube of MgCl2 for titration (D4545). The latter option may be necessary to determine optimal conditions for amplification.

Unit Definition

One unit will incorporate 10 nmol of total dNTPs into acid-precipitable DNA in 30 min at 74 °C.

No detailed specifications are available for this product.

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Code Description Colour Quality Level Manufacturer Feature Form Price Quantity
3582215 Taq DNA Polymerase from Thermus aquaticus, with 10x PCR reaction buffer containing MgCl2 colorless 200 SIGMA-ALDRICH hotstart: no, dNTPs included: no liquid
£1,021.82 (exc VAT) per EACH
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3582216 Taq DNA Polymerase from Thermus aquaticus, with 10x PCR reaction buffer containing MgCl2 colorless 200 SIGMA-ALDRICH hotstart: no, dNTPs included: no liquid
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3582217 Taq DNA Polymerase from Thermus aquaticus, with 10x PCR reaction buffer containing MgCl2 colorless 200 SIGMA-ALDRICH hotstart: no, dNTPs included: no liquid
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3582218 Taq DNA Polymerase from Thermus aquaticus, with 10x PCR reaction buffer containing MgCl2 colorless 200 SIGMA-ALDRICH hotstart: no, dNTPs included: no liquid
£205.07 (exc VAT) per EACH
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3582219 Taq DNA Polymerase from Thermus aquaticus, with 10x PCR reaction buffer containing MgCl2 colorless 200 SIGMA-ALDRICH hotstart: no, dNTPs included: no liquid
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