SYBR Green JumpStart Taq ReadyMix, for quantitative PCR, without MgCl2 in buffer

SYBR Green JumpStart Taq ReadyMix, for quantitative PCR, without MgCl2 in buffer

Application

SYBR®Green JumpStart™TaqReadyMix™ has been used:to prepare the real-time PCRmixturein the quantitative analysis of gene expression by real time, quantitativepolymerase chain reaction (qPCR)to determine the mRNA expression of nuclear factor erythroid 2 (NFE2)-related factor 2 (Nrf2) gene by qPCR

SYBR® Green JumpStart® Taq ReadyMix™ has been used:During RNA extraction, reverse transcription and Quantitative PCRDuring analysis of leptin and leptin receptor expression. (PCR and melting curve analyses were performed)For tissue RNA Isolation. (PCR and melting curve analyses were performed)in real-time PCR (RT-PCR) of reverse-transcribed cDNA template For qPCR amplifications using SYBRFor multiplex PCR

Features and Benefits

Delivers the benefits of antibody-inactivated hot-start PCR with SYBR Green detection in a ReadyMix ideal for high throughput applications; only primers and template are required JumpStart™ Taq DNA polymerase prevents amplification of non-specific products, resulting in increased efficiency and higher target yield SYBR® Green JumpStart™ Taq ReadyMix for SYBR based qPCR is formulated with MgCl2 or packaged with a separate vial for ease of optimization. ReadyMixes are compatible with tube- and plate-based instruments This master mix allows consistency from one reaction to the next Designed to minimize contamination Reduced primer dimers Reduced set-up time as compared to manual or wax HotStart® methods Allows for room temperature set-up and contains a fluorescent dye, which is ideal for qPCR applications

General description

SYBR® Green JumpStart™ Taq ReadyMix™ combines the performance enhancements of JumpStart Taq antibody for hot start PCR with SYBR Green I and the convenience of an easy-to-use ReadyMix solution. This ready-to-use mixture of SYBR Green I, JumpStart Taq DNA polymerase, 99% pure deoxynucleotides and reaction buffer is provided in a 2× concentrate for ease of use. Simply add 25 µL of the 2× mix to DNA template, primers and water. The JumpStart Taq antibody inactivates DNA polymerase at room temperature. When the temperature is raised above 70 °C in the first denaturation step of the cycling process, the complex dissociates and the polymerase becomes fully active. JumpStart Taq DNA polymerase prevents non-specific amplification resulting in more accurate CT values.

Legal Information

SYBR is a registered trademark of Life Technologies

Julabo is a registered trademark of Julabo USA, Inc.

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No

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Code Description Colour Manufacturer Compatibility Feature Form Price Quantity
3582458 SYBR Green JumpStart Taq ReadyMix, for quantitative PCR, without MgCl2 in buffer colorless SIGMA-ALDRICH for use with Roche LightCycler 480, for use with ABI 7700, for use with Eppendorf® Mastercycler ep realplex, for use with ABI StepOne, for use with Bio-Rad hotstart, dNTPs included liquid
£330.11 (exc VAT) per 100RXN
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3582459 SYBR Green JumpStart Taq ReadyMix, for quantitative PCR, without MgCl2 in buffer colorless SIGMA-ALDRICH for use with Roche LightCycler 480, for use with ABI 7700, for use with Eppendorf® Mastercycler ep realplex, for use with ABI StepOne, for use with Bio-Rad hotstart, dNTPs included liquid
£104.33 (exc VAT) per 20RXN
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3582460 SYBR Green JumpStart Taq ReadyMix, for quantitative PCR, without MgCl2 in buffer colorless SIGMA-ALDRICH for use with Roche LightCycler 480, for use with ABI 7700, for use with Eppendorf® Mastercycler ep realplex, for use with ABI StepOne, for use with Bio-Rad hotstart, dNTPs included liquid
£659.29 (exc VAT) per 400RXN
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