Streptomycin sulfate salt, powder, BioXtra, suitable for mouse embryo cell culture

Descriptions

Application

Streptomycin is used to study mechanisms of streptomycin resistance and is often used together with penicillin and other agents to inhibit bacterial contamination in cell culture applications. It is recommended for use in molecular biology applications at 25-50µg/mL, in cell culture applications at 100 mg/L and in embryo culture at 50 mg/L.

Biochem/physiol Actions

Mode of Action: Streptomycin acts by inhibiting prokaryote protein synthesis by binding to the S12 protein of the 30S ribosomal subunit, thus preventing the transition from imitation complex to chain-elongating ribosome. This causes miscoding or inhibits initiation.Mode of Resistance: A mutation in rpsL, a gene for S12 ribosomal protein, prevents binding of streptomycin to the ribosome. An aminoglycoside phosphotransferase also inactivates streptomycin.Antimicrobial spectrum: Streptomycin acts against gram-negative and gram-positive bacteria.

Caution

Solutions can be stored at 2-8°C for up to a month or at -20°C for more extended periods. At 37°C, solutions are stable for three days.

General description

Chemical structure: aminoglycoside

Packaging

5, 50 g in poly bottle

Preparation Note

Stock solutions should be prepared with sterile water at concentrations between 20-25 mg/ml.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Pack	InChI key	InChI	Manufacturer	Product Line	Form	Price		Quantity
3572238	Streptomycin sulfate salt, powder, BioXtra, suitable for mouse embryo cell culture	200	50G	QTENRWWVYAAPBI-YZTFXSNBSA-N	1S/2C21H39N7O12.3H2O4S/c21-5-21(36,4-30)16(40-17-9(26-2)13(34)10(31)6(3-29)38-17)18(37-5)39-15-8(28-20(24)25)11(32)7(27-19(22)23)12(33)14(15)35;31-5(2,3)4/h2	SIGMA-ALDRICH	BioXtra	powder	£269.80 (exc VAT) per 50G		- +
3572239	Streptomycin sulfate salt, powder, BioXtra, suitable for mouse embryo cell culture	200	5G	QTENRWWVYAAPBI-YZTFXSNBSA-N	1S/2C21H39N7O12.3H2O4S/c21-5-21(36,4-30)16(40-17-9(26-2)13(34)10(31)6(3-29)38-17)18(37-5)39-15-8(28-20(24)25)11(32)7(27-19(22)23)12(33)14(15)35;31-5(2,3)4/h2	SIGMA-ALDRICH	BioXtra	powder	£68.42 (exc VAT) per 5G		- +