SSC Buffer, powder blend

Application

Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.

Suitable for: Preparing nucleic acids for hybridization Hybridization buffer for Northern and Southern blots Washing buffer for Northern and Southern blots

Components

Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

Features and Benefits

Multi-purpose use in hybridizations Easily reconstituted and diluted to any concentration needed

General description

SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

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Code Description Quality Level Pack pH Manufacturer Storage Temp. Solubility Form Price Quantity
3577450 SSC Buffer, powder blend 200 1L 6.8-7.2 (20-25 °C, 263 mg/L in water) SIGMA-ALDRICH room temp water: 263 mg/mL, clear, colorless powder blend
£121.79 (exc VAT) per 1L
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3577451 SSC Buffer, powder blend 200 - 6.8-7.2 (20-25 °C, 263 mg/L in water) SIGMA-ALDRICH room temp water: 263 mg/mL, clear, colorless powder blend
£470.54 (exc VAT) per EACH
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