SILU(TM)MAB K1 STABLE-ISOTOPE LABELED

SILU(TM)MAB K1 STABLE-ISOTOPE LABELED

Analysis Note

SILuMAb K1 Heavy ChainEVQLVESGGGLVQPGGSLRLSCVASGFTLNNYDMHWVRQGIGKGLEWVSKIGTAGDRYYAGSVKGRFTISRENAKDSLYLQMNSLRVGDAAVYYCARGAGRWAPLGAFDIWGQGTMVTVSSASTKGPSVFPLAPSSKSTSGGTAALGCLVKDYFPEPVTVSWNSGALTSGVHTFPAVLQSSGLYSLSSVVTVPSSSLGTQTYICNVNHKPSNTKVDKKVEPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPG SILuMAb K1 Light ChainQSALTQPRSVSGSPGQSVTISCTGTSSDIGGYNFVSWYQQHPGKAPKLMIYDATKRPSGVPDRFSGSKSGNTASLTISGLQAEDEADYYCCSYAGDYTPGVVFGGGTKLTVLTVAAPSVFIFPPSDEQLKSGTASVVCLLNNFYPREAKVQWKVDNALQSGNSQESVTEQDSKDSTYSLSSTLTLSKADYEKHKVYACEVTHQGLSSPVTKSFNRGECTarget overlap areas are underlinedPackage size based on protein content determined by A280 using an extinction coefficient (E0.1%) of 1.4

QuantitativeMRM settings provided (xls)

Application

SILu™ MAB K1 Stable-Isotope Labeled Universal Monoclonal Antibody has been used to spike peptide samples to assess preparation reproducibility.

Features and Benefits

Universal Peptide Sequence LocationFNWYVDGVEVHNAKHeavy Chain (IgG1)VVSVLTVLHQDWLNGKHeavy Chain (IgG1, IgG3, IgG4)GFYPSDIAVEWESNGQPENNYKHeavy Chain (IgG1, IgG4)SGTASVVCLLNNFYPRLight Chain (kappa)VDNALQSGNSQESVTEQDSKLight Chain (kappa)DSTYSLSSTLTLSKLight Chain (kappa)SILu™Mab has been validated as an internal standard for quantitation of relevant biotherapeutics in a complex biological matrix by MRM-based LC-MS/MS. •SILu™Mab yielded reproducible, linear curves from 0.1 μg/mL to 1000 μg/mL without enrichment or depletion. •Good agreement was observed between multiple peptides derived from the same target. •Label incorporation was determined to be >98% by mass spectrometry. •Sequence coverage was confirmed by peptide mapping.

Legal Information

SILu is a trademark of Sigma-Aldrich Co. LLC

This product is licensed under U.S. Patent No. 7,396,688 and foreign counterparts from E. I. du Pont de Nemours and Company. The purchase of this product conveys to the buyer the nontransferable right to use the purchased amount of the product for research and development only, including services for a third party for consideration. The buyer cannot sell or otherwise transfer this product, its components or materials made using this product or its components to a third party. Information about licenses for excluded uses is available from: E. I. du Pont de Nemours and Company; Attn: Associate Director, Commercial Development; DuPont Experimental Station E268; 200 Powdermill Rd.; Wilmington, DE 19803; 1-877-881-9787 (voice), 1-302-695-1437 (fax), licensing@dupont.com.

Physical form

Supplied as a lyophilized powder containing phosphate buffered saline

Preparation Note

SILu™Mab design is optimized to be used as an internal standard for quantitation of monoclonal antibodies as well as Fc-fusion therapeutics

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Code Description Quality Level Manufacturer Storage Temp. Assay Price Quantity
3587194 SILU(TM)MAB K1 STABLE-ISOTOPE LABELED 200 SIGMA-ALDRICH −20°C ≥90% (SDS-PAGE)
£501.43 (exc VAT) per 100UG
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3587195 SILU(TM)MAB K1 STABLE-ISOTOPE LABELED 200 SIGMA-ALDRICH −20°C ≥90% (SDS-PAGE)
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