S-Gal sodium salt, reagent for selection of recombinant bacterial clones

S-Gal sodium salt, reagent for selection of recombinant bacterial clones

Application

S-Gal, sodium salt is a patented water-soluble, autoclavable chromogenic substrate for β-galactosidase that is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Caution

For black color development to occur, ferric ion must be present. Although we recommend ferric ammonium citrate (500 mg/L of media), other ferric compounds can be used to provide this requirement, depending upon your particular system.

Features and Benefits

When S-Gal is cleaved by β-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies. S-Gal, Sodium salt is water soluble, eliminating the need for solvents such as dimethyl formamide. S-Gal is also autoclavable and can be added to your medium of choice prior to autoclaving. S-Gal is not light sensitive and does not require any protection from light sources.

More intense color contrast than X-gal Water-soluble and autoclavable for easiest use Excellent for use in automated colony counters No need to make stock solutions

General description

S-Gal® (sodium salt) is an autoclavable, water-soluble, chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. S-gal® is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Legal Information

S-GAL is a registered trademark of Sigma-Aldrich Co. LLC

Linkage

Ferric Ammonium Citrate (F5879), LB Agar (L2897), IPTG (Isopropyl β-D-thiogalactopyranoside, I6758), Ampicillin (A2804), Kanamycin (K0879), Chloramphenicol (C7795), Tetracycline (T8032)

Other Notes

The ferric or Fe3+ ion is required for color development and must be added to any S-Gal®formulation. A medium prepared with S-Gal® is moderately dark due to the presence of ferric ammonium citrate. This darker background often provides enhanced contrast for automated colony counting or isolation.

More water soluble version, soluble >50 mg/mL.

Packaging

100 mg in poly bottle

1, 5 g in poly bottle

Principle

When S-Gal® is cleaved by ß-galactosidase, the resulting product will chelate ferric ion to create a black, insoluble precipitate. Lac+ colonies grown in the presence of S-Gal® and ferric ion turn an intense black color, allowing for easy differentiation between lac+ and lac- colonies.

Reconstitution

Stock solutions of S-Gal® (sodium salt) can be made by dissolving 50mg/ml in water, dimethyl formamide (DMF) or DMSO. Filter sterilize and store at -20C. Add S-gal® (300 mg/L from stock solution or powder) and Ferric Ammonium Citrate (500mg/L) to agar media prior to autoclaving.

No detailed specifications are available for this product.

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Code Description Quality Level Manufacturer Storage Temp. Solubility Form Assay Price Quantity
3577230 S-Gal sodium salt, reagent for selection of recombinant bacterial clones 200 SIGMA-ALDRICH room temp H2O: 100 mg/mL
£153.84 (exc VAT) per 100MG
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3577231 S-Gal sodium salt, reagent for selection of recombinant bacterial clones 200 SIGMA-ALDRICH room temp H2O: 100 mg/mL
£1,057.33 (exc VAT) per 1G
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3577232 S-Gal sodium salt, reagent for selection of recombinant bacterial clones 200 SIGMA-ALDRICH room temp H2O: 100 mg/mL
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