ROCHE Pyruvate Kinase (PK)from rabbit muscle

Descriptions

Application

Pyruvate kinase has been used to measure ATPase activity and in the determination of adenylate concentration.

Biochem/physiol Actions

Pyruvate kinase catalyzes the irreversible conversion of P-enolpyruvate and ADP to pyruvate and ATP with the utilization of a proton. The first step is the transfer of phosphate group from P-enolpyruvate to ADP with the formation of bound enolate of pyruvate and ATP. In the second step, a proton is added to enolate to generate the keto form of pyruvate. Apart from this, the enzyme exhibits other activities, such as ATP- and bicarbonate-dependent ATPase, phosphorylation of fluoride and hydroxylamine, ATP-dependent phosphorylation of glycolate, and decarboxylation of oxaloacetate.

General description

ATP:pyruvate 2-O-phosphotransferasePyruvate kinase has a molar mass of 237,000 and exists as a tetramer. Each polypeptide chain of this tetramer has a molar mass of 57,200. The enzyme contains two identical catalytic particles called protomers. Each of these protomers contains two polypeptide chains. Each protomer contains one site each for Mn²⁺ and phosphoenolpyruvate.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Physical form

Suspension in 3.2 M ammonium sulfate solution, pH approximately 6

Preparation Note

Activator: PK requires Mg²⁺ (or Mn²⁺, Co²⁺) and K⁺ (or NH⁴⁺, Rb⁺) for full activity.

Quality

Contaminants: ﹤0.001% GK, ﹤0.002% “NADH oxidase”, and ATPase, each, ﹤0.01% enolase, LDH, and myokinase, each

Specifications

No detailed specifications are available for this product.

Downloads

There are no downloads for this product.

Code	Description	Quality Level	Storage Temp.	Form	Price		Quantity
2795405	ROCHE Pyruvate Kinase (PK)from rabbit muscle	100	2-8°C	suspension	£111.43 (exc VAT) per EACH		- +
2795406	ROCHE Pyruvate Kinase (PK)from rabbit muscle	100	2-8°C	suspension	£588.20 (exc VAT) per EACH		- +