ROCHE Pwo Superyield DNA Polymerase

ROCHE Pwo Superyield DNA Polymerase

Application

Pwo Super Yield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with a newly optimized buffer system. Pwo SuperYield DNA Polymerase is used for the amplification of DNA with the intent to sequence the amplification product or to clone the product (e.g., for the expression of the gene product). The high fidelity of this enzyme makes it particularly suitable for: High fidelity PCRSite-directed mutagenesisCloningGene expressionStudy of allelic polymorphism in individual RNA transcriptsCharacterization of rare mutations in tissueCharacterization of the allelic stage of single cells or single DNA molecules

Features and Benefits

Pwo SuperYield DNA Polymerase yields more high fidelity PCR product. The optimized buffer delivers superior results. Amplify fragments up to 3kb. A GC-RICH Solution is included for difficult templates. Higher yield and 18-fold higher fidelitycompared to Taq DNA Polymerase. High performance with difficult templates.The GC-RICH Solution is for GC-rich PCR. Reduce working steps in cloning.Perform digests directly in Pwo SuperYield PCR mix.

General description

Pwo SuperYield DNA Polymerase combines the recombinant enzyme Pwo DNA Polymerase with an optimized buffer system. This buffer system enhances the enzymatic properties of the polymerase, resulting in higher yields of the amplification reaction without changing the fidelity of DNA synthesis. This enzyme delivers excellent results due to its enzyme design and optimized buffer system. Amplify fragments up to 3 kb - even longer amplicons are possible from simple templates.Pwo SuperYield DNA Polymerase exhibits increased thermal stability with a half life of greater than 2 hours at +100 °C compared to Taq DNA Polymerase with a half life of less than 5 min at this temperature.

Pwo SuperYield DNA Polymerase, originally isolated from Pyrococcus woesei, is a processive 5′ →3′ DNA polymerase with 3′ →5′ exonuclease proofreading activity; 5′ →3′ exonuclease activity is not detectable.The enzyme accepts modified nucleotides for efficient labeling of nucleic acids by PCR.PCR products are blunt-ended directly useable for blunt-end ligation.Using the magnesium-containing reaction buffer supplied, the final MgCl2 concentration is 1.5mM.

Legal Information

Use of this product is covered by US patent claims and corresponding patent claims outside the US. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patent claims require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.﹤/ br>NOTICE TO PURCHASER: LIMITED LICENSE﹤br /

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Code Description Quality Level Technique(s) Parameter Feature Storage Temp. Price Quantity
2795255 ROCHE Pwo Superyield DNA Polymerase - 500 U 100 PCR: suitable 72 °C optimum reaction temp. hotstart: no, Difficult Templates/Specialty Enzymes PCR, High Fidelity PCR, dNTPs included: no −20°C
£785.57 (exc VAT) per EACH
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2795256 ROCHE Pwo Superyield DNA Polymerase - 100 U 100 PCR: suitable 72 °C optimum reaction temp. hotstart: no, Difficult Templates/Specialty Enzymes PCR, High Fidelity PCR, dNTPs included: no −20°C
£189.43 (exc VAT) per EACH
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