ROCHE CDP-Star ready-to-useDisodium 2-chloro-5-(4-methoxyspiro (12-dioxetane-32-(5-chloro)tricyclo[3

Descriptions

Application

Used for the nonradioactive detection of labeled nucleic acid probes on membrane blots: Southern blotNorthern blotDot blotColony and plaque hybridizationGel shift assays

Features and Benefits

CDP-Star is a chemiluminescent substrate for alkaline phosphatase that enables fast, ultra-sensitive detection of biomolecules by producing extremely rapid visible light. Light emission is recorded on X-ray film or on luminescence imager systems. Save time with a fast, ultra-sensitive substrate. Easily strip and reprobe membranes. Take multiple exposures of up to two days for each experiment. Reuse the substrate by filtering and storing in sodium azide.

General description

Contents25 mM solution (12.38 mg/ml), 100x concentration

Legal Information

CDP-Star is a registered trademark of Tropix, Inc.

Other Notes

For life science research only. Not for use in diagnostic procedures.

First exposure to X-ray film should be 5-15 minutes. Examine film and then determine other exposure times based on initial result. No preincubation step required. Apply substrate quickly to avoid gray shadows from substrate drops and drying of membrane (leads to uneven, high background). Do not use plastic wrap to cover blot; use hybridization bags, acetate sheet protectors, or two sheets of transparent film. Exposure times are shorter than with CSPD. Do not use nitrocellulose membranes. Repeat exposures can be made up to two days after the addition of substrate.

Preparation Note

Storage conditions (working solution): Washing Buffer: 15 to 25 °C, stableMaleic Acid Buffer: 15 to 25 °C, stableDetection Buffer: 15 to 25 °C, stableBlocking Solution: always prepare freshlyAntibody Solution: 2 to 8 °C

Quality

Purity: Purified by HPLC.

Specifications

No detailed specifications are available for this product.

Downloads

There are no downloads for this product.

Code	Description	Quality Level	InChI key	InChI	Technique(s)	Further Info	Storage Temp.	Form	Assay	Price	Quantity
2795690	ROCHE CDP-Star ready-to-useDisodium 2-chloro-5-(4-methoxyspiro (12-dioxetane-32-(5-chloro)tricyclo[3	100	UKWLRLAKGMZXJC-VCCHNUGISA-L	1S/C18H21Cl2O7P.2Na/c1-24-18(14-6-13(2-3-15(14)19)25-28(21,22)23)17(26-27-18)11-4-10-5-12(17)9-16(20,7-10)8-11;;/h2-3,6,10-12H,4-5,7-9H2,1H3,(H2,21,22,23);;/q;	-	-	-	solution	>98%	£635.14 (exc VAT) per EACH	- +
2795718	ROCHE CDP-Star ready-to-useDisodium 2-chloro-5-(4-methoxyspiro (12-dioxetane-32-(5-chloro)tricyclo[3	100	UKWLRLAKGMZXJC-VCCHNUGISA-L	1S/C18H21Cl2O7P.2Na/c1-24-18(14-6-13(2-3-15(14)19)25-28(21,22)23)17(26-27-18)11-4-10-5-12(17)9-16(20,7-10)8-11;;/h2-3,6,10-12H,4-5,7-9H2,1H3,(H2,21,22,23);;/q;	-	-	-	solution	>98%	£1,043.25 (exc VAT) per EACH	- +
2795766	ROCHE CDP-Star ready-to-useDisodium 2-chloro-5-(4-methoxyspiro (12-dioxetane-32-(5-chloro)tricyclo[3	100	-	-	dot blot: suitable, Southern blotting: suitable, Northern blotting: suitable	Disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2&prime;-(5&prime;-chloro)tricyclo[3.3.1.13,7 ]decan}-4-yl)-1-phenyl phosphate	2-8°C	solution	>98%	£547.39 (exc VAT) per EACH	- +