Ribonuclease B from bovine pancreas, BioReagent, >=50 Kunitz units/mg protein, >=80% (SDS-PAGE)

Application

Ribonuclease B from bovine pancreas is used in the digestion of RNA during cell cycle platform analysis.

Biochem/physiol Actions

Native RNase BS generated by subtilisin digestion of native RNase B comprising of amino acid residues 21-124 of RNase B, is sensitive to PNGase F digestion. Intramolecular N-glycans of bovine pancreatic RNase B function like chaperone. RNase B is found to be much faster than RNase A, while RNase A is liable to aggregate during regeneration. The stimulatory effect of Asn-oligosaccharide (which corresponds to the most predominant sugar chain of RNase B) reveals that the N-glycans of RNase B facilitates the transformation of bulky intermediates into folded, compact species.

Packaging

100 mg in poly bottle

Package size based on protein content

500 µg in poly bottle

Preparation Note

Purified by affinity chromatography

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Code Description Quality Level Manufacturer Product Line Storage Temp. Form Assay Price Quantity
3587310 Ribonuclease B from bovine pancreas, BioReagent, >=50 Kunitz units/mg protein, >=80% (SDS-PAGE) 200 SIGMA-ALDRICH BioReagent −20°C
£888.64 (exc VAT) per 100MG
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3587311 Ribonuclease B from bovine pancreas, BioReagent, >=50 Kunitz units/mg protein, >=80% (SDS-PAGE) 200 SIGMA-ALDRICH BioReagent −20°C
£62.40 (exc VAT) per 500UG
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