Ribonuclease A from bovine pancreas, Type I-AS, 50-100 Kunitz units/mg protein

Descriptions

Analysis Note

Protein determined by E.

Application

RNase A is used to remove RNA from DNA plasmid and genomic DNA preparations and protein samples. RNase A is also used in RNA sequence analysis and protection assays. RNase A has been used as a tool for computer-aided drug design. RNase A supports the analysis of RNA sequences. RNase A hydrolyze RNA contained in protein samples. Purification of DNA is supported by RNase A.

Biochem/physiol Actions

Ribonuclease A is an endoribonuclease that cleaves single stranded RNA after pyrimidine nucleotides. It attacks at the 3’; phosphate end. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts.

Features and Benefits

Our highly stable Ribonuclease A, RNase A, is suitable for removal of RNA, RNA sequencing, and DNA purification.

General description

RNase A, Ribonuclease A, is an endoribonuclease that cleaves the phosphodiester bonds of single strand RNA after pyrimidine nucleotides. It attacks at the 3′ phosphate end (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG). The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. In contrast to RNase B, it is not a glycoprotein. Ribonucleases do not hydrolyze DNA, because the DNA lacks 2′-OH groups essential for the formation of cyclic intermediates. RNase A can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. RNAse is inhibited in the presence of heavy metal ions. RNase is also inhibited competitively by DNA.

Packaging

1, 5, 10 g in poly bottle

100, 500 mg in poly bottle

Preparation Note

Salt fractionated and chromatographically purified.

Specifications

No detailed specifications are available for this product.

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Code	Description	Quality Level	Manufacturer	Storage Temp.	Impurities	Form	Price	Quantity
3581279	Ribonuclease A from bovine pancreas, Type I-AS, 50-100 Kunitz units/mg protein	300	SIGMA-ALDRICH	−20°C	salt, essentially free	lyophilized powder	£107.53 (exc VAT) per 100MG	- +
3581280	Ribonuclease A from bovine pancreas, Type I-AS, 50-100 Kunitz units/mg protein	300	SIGMA-ALDRICH	−20°C	salt, essentially free	lyophilized powder	Click Add to Quote for price and delivery	- +
3581281	Ribonuclease A from bovine pancreas, Type I-AS, 50-100 Kunitz units/mg protein	300	SIGMA-ALDRICH	−20°C	salt, essentially free	lyophilized powder	£552.96 (exc VAT) per 1G	- +
3581282	Ribonuclease A from bovine pancreas, Type I-AS, 50-100 Kunitz units/mg protein	300	SIGMA-ALDRICH	−20°C	salt, essentially free	lyophilized powder	£324.54 (exc VAT) per 500MG	- +
3581283	Ribonuclease A from bovine pancreas, Type I-AS, 50-100 Kunitz units/mg protein	300	SIGMA-ALDRICH	−20°C	salt, essentially free	lyophilized powder	Click Add to Quote for price and delivery	- +