REDExtract-N-Amp Blood PCR Kit

REDExtract-N-Amp Blood PCR Kit

Application

REDExtract-N-Amp™ Blood PCR Kit has been used to extract genomic DNA from blood samples, peripheral blood leukocytes, endocervical swab eluates of mice and parasites. It has also been used to perform polymerase chain reaction (PCR) using oligonucleotide primers.

Features and Benefits

Efficient 8 minute prep allows greater speed and throughput No need for organic extraction, centrifugation or alcohol precipitation Hot start antibody for highly specific PCR amplification of genomic DNA Can be used with whole blood or blood cards Extract stable at 4 °C for at least 6 months Compatible with any format (single tube, 96-well, etc.) Simple, 3 step procedure with no special equipment required REDExtract-N-Amp™ requires no loading buffers or tracking dyes required for gel analysis PCR products are suitable for direct cloning and sequencing

General description

The REDExtract-N-Amp™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR (polymerase chain reaction). This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification. The REDExtract-N-Amp Blood PCR Kit contains a tracking dye that allows for convenient direct loading of PCR reactions onto agarose gels for analysis.

Legal Information

Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.

REDExtract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

ReadyMix is a trademark of Sigma-Aldrich Co. LLC

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche

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Code Description Colour Quality Level Manufacturer Technique(s) Feature Storage Temp. Price Quantity
3582511 REDExtract-N-Amp Blood PCR Kit, sufficient for 100 extractions, sufficient for 100 amplifications red 200 SIGMA-ALDRICH PCR: suitable hotstart, dNTPs included −20°C
£385.82 (exc VAT) per 1KIT
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3582514 REDExtract-N-Amp Blood PCR Kit, sufficient for 100 extractions, sufficient for 500 amplifications red 200 SIGMA-ALDRICH - hotstart, dNTPs included −20°C
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3582515 REDExtract-N-Amp Blood PCR Kit, sufficient for 1000 extractions, sufficient for 1000 amplifications red 200 SIGMA-ALDRICH PCR: suitable hotstart, dNTPs included −20°C
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3582516 REDExtract-N-Amp Blood PCR Kit, sufficient for 10 extractions, sufficient for 10 amplifications red 200 SIGMA-ALDRICH PCR: suitable hotstart, dNTPs included −20°C
£48.05 (exc VAT) per 1KIT
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